Dr. Shakira Ghazanfar got her Ph. D. degree in Microbiology/ Probiotic Specialists. She is working as Senior Scientific Officer, Functional Genomics and Bioinformatics, National Agricultural Research Centre, Pakistan since 2010. She was regarded among the pioneers, who have initiated animal probiotic product preparation work based on research to improve livestock productivity in Pakistan by using local isolated microbial strains. She worked on various projects related to the improve livestock productivity based on probiotic. More than 500 sanger sequenced bacterial strains and 10 whole genomes sequencing based FAO approved bacteria isolated from local animal gut/soil/dairy items etc. and submitted their sequencing in to NCBI. She initiated the project for the Establishment of NIGAB-Microbial Culture Collection (NMCC) at NARC. MNCC is a microbial gene bank for the preservation of the bacterial strains for future use in teaching, research and industrial application. Currently She has more than 300 microbial isolates in the NMCC including Pediococcus pentosaceus: Bacillus tequilensis, Lactobacillus agilis, Lactobacillus delbrueckii: Pediococcus acidilactici, Enterococcus faecalis, Weissella cibaria, Weissella confusa, Weissella bombi, Lactobacillus plantarum, Lactobacillus rhamnosus etc. At present, her prime research interests are: Target Probiotic preparation by using Next generation sequencing tools; Food Safety (food-borne illness associated pathogens, nano-active packaging), Nano-medicine (multi-drug resistance related diseases). She is an active researcher and produced many publications in well reputed journals and conferences and published many research papers and book chapters and engaged in research supervising multiple students at PhD, Graduate and Under-graduate level.